Flow Cytometry Work Cell
The Automated Flow Cytometry work cell provides scientists with an automated sample acquisition system for screening cells and beads in solution. This integrated platform combines the Intellicyt iQue™ Screener with our PlateCrane EX microplate robot arm.
Combining the PlateCrane EX with the iQue Screener provides the user with a powerful system for screening large libraries for those that act as desired in various cell and bead-based assays, on a bench top or in a hood.
Hudson’s cost-effective workcell offers small footprints for mobility and flexibility, and easy-to-useSoftLinx software for data tracking and scheduling. SoftLinx communicated directly with Intellicyt’s ForeCyt™ software and automatically loads and executes stored protocols. SoftLinx makes it easy to expand your system with the addition of other instrumentation, such as bar code scanners, liquid handlers, heating and cooling nests, and plate sealers.
The Flow Cytometry work cell supports a wide range of cell and bead-based assays. A few general areas of research supported by this system include:
- Small Molecule Cell-Based Drug Discovery
- Antibody Engineering
- Phenotypic Screening
- in Vitro Toxicology Screening
See the Intellicyt website for more details on specific applications.
iQue Specifications – Click here for PlateCrane Specification
| SYSTEM | |
| Dimensions (W x D x H) | 99 x 63 x 66 cm (39 x 25 x 26 inches) |
| Weight | 82 kg (180 lb) |
| Sampling | Manual and with autosampler. Autosampler accommodates 96- and 384-well plates with plate recall auto settings. 1536-well is a planned future capability compatible with current hardware. |
| Minimum Volume Requirements | Zero dead volume, 1 µL sampled volume, 10 µL assay volume (96-well plates) |
| Screening Detection Method | Flow cytometry |
| Event Rate Detection | 10,000/sec |
| Sensitivity Range | 6+ decades |
| Gain and Voltage Settings | Automatic |
| Laser Excitation | Solid state lasers: blue (488 nm) and red (633 nm) |
| Multicolor Detection | 4 simultaneous color channels using PMTs |
| Label-free Object Detection | 2 light scatter channels for size granularity |
| Sensitivity | 7 decade dynamic range using fixed optics |
| Minimum Plate Processing Speed | 3 minutes for one 96-well plate, 12 minutes for one 384-well plate |
| SOFTWARE | |
| Enterprise Data Software | Standard |
| Software Features | Standard: normalization, dynamic linked gating, interactive heat maps, 1D and 2D plot settings, custom PDF data report, well scan, unified work space, custom metrics, automated cleaning, one data file per plate, templates, advanced screening software. File size per 96-well plate: 70-100 MB. File export: FCS, CSV and ForeCyt plate formats. |
| Computer Requirements | Windows 7, 64-bit |
| SAFETY | |
| CE Compliance | Yes |